DIAGNOSTIC STUDY OF TOXOPLASMA GONDII IN BLOOD DONOR AND APPLICANT FOR MARRIAGE OF THI-QAR UNIVERSITY-IRAQ BY REAL- TIME PCR

Abstract

A rare intracellular parasite called Toxoplasma gondii infects a sizable fraction of the global population, although it seldom results in serious illness. Venous blood samples were obtained from 300 individuals (160 blood donors and 140 marriage applicants) who appeared to be in good health. The individuals ranged in age from 17 to 42 years old and had been treated for toxoplasma gondii infection in Thi-Qar for both sexes between October 2023 and the end of January 2024 using an enzyme-linked immunosorbent assay (ELISA). was employed to detect the B1 gene of T.gondii DNA using Real-Time Polymerase Chain Reaction (RT-PCR) and assess the existence of anti-Toxoplasma IgM and IgG antibodies .The findings demonstrated that, when using the ELISA antibody IgG, the overall toxoplasma gondii positive rate was 33%, and when utilizing the antibody for blood donors, it was 3%. For blood donors, the positive rate for antibodies was 28%, and for marriage applicants, the positive rate for antibodies was 5%.Twenty positive cases of anti-Toxoplasma antibodies were found in any positive blood sample with IgG and IgM, respectively. The ability to detect recent or active toxoplasma in applicants through marriage and blood donation in real time is advantageous.