A STUDY OF THE RELATIONSHIP BETWEEN PC-1 GENE POLYMORPHISM AND INSULIN RESISTANCE IN PATIENTS WITH DIABETES TYPE 2 IN THI-QAR GOVERNORATE

Abstract

The research study was carried out between December and May 2023 at the Biotechnology Research Unit of Thi-Qar at Mazaya University College. was the current study aimed to identify the genetic polymorphisms of 120 samples to investigate the possible association of PC-1 gene polymorphism with the diabetes mellitus. The observed PCR amplicons were subjected to the DNA sequencing procedure. The discovered variants were then localized based on where they were located within the reference genomic DNA sequences. The recent findings showed that the samples under investigation had genetic variations that were unevenly distributed. Besides, the rs1044498, A248C,also the high frequency variant is usually detected in three polymorphic modes, AA, AC, CC. Homozygous AA pattern discovered in the majority of patients (62.5%) and normal specimens (60%). However, each observed SNP was taken a particular pattern of variation differ from its mate in terms of the investigated samples. Ultimately, a statistical analysis is required to evaluate the possible association of the SNPs is tested samples, with the diabetes mellitus. As a result, the PC1 polymorphism was found to be associated with type 2 diabetes mellitus and insulin resistance in the current investigation.. Mutations in the PC-1 gene were recorded in the bank gene(NCBi) with accession numbers. LC768831, LC768832, LC768833, LC768834, LC768835. LC768836, LC768837 LC768838, LC768839, LC768840, LC768841, LC768842, LC768843.