SPECTROPHOTOMETRIC DETERMINATION OF PARACETAMOL DRUG BY USE ETHYL 2-(4-NITROPHENYL) ACETATE (M9) LAP REAGENT PREPARED

Abstract

Sensitive, fast, accuracy spectrophotometric method developed to determination of paracetamol drug depend on reaction between drug and sodium nitrite solution in acid medium to produced dizonium salt and react with M9 reagent in basic solution, the final product of the reaction was formed, which was immediately yellow stable for more than 60 minutes, which is a sufficient time to measure many samples. The highest absorption was given at 429 nm, where it follows Beer's law in the range of concentrations between 5 - 40 μg/ml. The molar absorption coefficient 3177.05 litres. mol-1.cm-1 and Sandel sensitivity 0.0476 µg. cm-2, r= 0.9988, LOD was 0.1413 µg/ml, and LOQ was 0.4281 µg/ml. and the method was successfully applied to paracetamol in pharmaceutical preparations.