BIOTECHNOLOGICAL STUDY OF THE ANTIBACTERIAL ACTIVITY OF ESCHERICHIA COLI AND OPTIMIZATION PROCEDURES FOR SILVER NITRATE NANOPARTICLES MANUFACTURED USING LAURUS NOBILIS EXTRACT

Abstract

In this work, a nano-device was used to create an aqueous extract of powdered bay leaves (Laurus nobilis) with silver nitrate. Various quantities of this extract were made using particles of silver nitrate (1\0.5\0.25\0.01\0.1). Its efficiency increases with decreasing extract concentration (e.g., 0.01 mm), and decreases with increasing extract concentration (1 mm). Chemical, physical, and laboratory testing were used to determine the plant's active component content. Escherichia coli, Klebsiella, and pseudomonas were among the organisms that were tested for antibacterial activity using the extract. use a MaC conky agar Petri plate for implantation. Since the average diameter of the inhibition zones clearly increased with increasing concentration of each plant extract against the bacteria, the study's findings regarding the inhibitory effectiveness of Laurus nobilis extracts varied based on the type of extract and the type of bacteria. As the concentration of each plant extract against the bacteria increased, there was a noticeable rise in the average width of the inhibitory zones. When tested against laboratory bacteria, the aqueous extract's inhibitory zones had average widths of 24-48 mm. It was evident from the average inhibitory zone widths that the extract originated from plant leaves. Since it showed the greatest rates of inhibition on one kind of bacteria, Escherichia coli, and the lowest rates on Klebsiella bacteria, laurel with silver nitratPe was obviously beneficial.