Biochemical Changes in Blood after Experimental Osteoporosis in Rats

Abstract

Summary: in order to study biochemical changes in the blood in osteoporosis after gastric resection under experimental conditions, female Wistar rats were resected by the Billrot-II method. The concentrations of calcium, phosphorus, magnesium and alkaline phosphatase in their blood serum were determined. Resection of the stomach of rats in the conditions of the experiment using the Billrot-II method led to biochemical changes in the blood serum.

Purpose of the study: study of biochemical changes in the blood in osteoporosis after gastric resection in rats under experimental conditions.

Material and methods: 32 mongrel white male rats weighing 170-200 g were selected for the experiment. Of these, 15 rats were divided as a control group and 17 as an experimental group. The rats of the experimental group underwent gastric resection using the Billrot-II method, and after three months the levels of calcium, phosphorus, magnesium and alkaline phosphatase in the blood serum were studied.

Research results: before the start of the experiment and on days 30, 60 and 90, the body weight of the animals of both groups was measured. After the operation, there was a decrease in the weight of rats compared to the control group until the end of the experiment. Biochemical changes were detected in the blood serum of operated rats.

Conclusion: the experience allowed us to conclude that after resection of the stomach of animals by the Billroth-II method, the condition of osteoporosis is caused by a violation of the digestive process, and accordingly, biochemical changes in the blood serum are observed.