DETERMINATION OF SULFATHIAZOLES SPECTROPHOTOMETRICALLY BY THE OXIDATIVE COUPLING METHOD USING THE COUPLING REAGENT

Abstract

This chapter includes the use of a simple and sensitive spectrophotometric method for the determination of sulfathiazoles using N,N-DMPPDAAs a coupling reagent in the presence of potassium periodate as an oxidizing agent to form a violet dye dissolved in water, it gave the highest absorption at the wavelength of 554 nm, the molar absorption value was 4161.699 l/mol.cm, and the Sandel significance value was 0.06134 micrograms/cm2, and the limits of Beer’s law were in the range of 3.125-50 micrograms. /ml of pure sulfathiazole in a final volume of 20 ml, the value of the percent recovery rate is 99.65, the value of the relative standard deviation is between 0.0018 - 0.0019%, the value of the detection limit is 0.995 micrograms/ml, and the quantitative limit is 3.319 micrograms/ml. This proposed method was successfully applied to estimate sulfathiazoles. In pharmaceutical preparations, it is in liquid form.